E-ISSN: 2619-9467

Contents    Cover    Publication Date: 11 Aug 2020
Year 2020 - Volume 30 - Issue 2

Open Access

Peer Reviewed

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The Beneficial Effects of Vitamin D3 Against Trichloroethylene Toxicity in Rat Ovaries

J Clin Obstet Gynecol. 2020;30(2):65-72
DOI: 10.5336/jcog.2020-75002
Article Language: EN
Copyright Ⓒ 2020 by Türkiye Klinikleri. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Objective: Vitamin D3 is an active metabolite of vitamin D and has been reported to have anti-oxidative effects due to inhibition of lipid peroxidation in some tissues both in vitro and in vivo studies. The current study was carried out to assess the protective effects of vitamin D3 on experimental trichloroethylene (TCE) toxicity in rat ovaries by using immunohistochemical detection of malondialdehyde (MDA) in tissues as well as histopathological scores' computation. Material and Methods: Twenty-four healthy rats were randomly divided into four equivalent groups: control (sham-operated), TCE+vitamin D3 (1000 mg/kg/day and 50 IU/day, in that order), TCE (1000 mg/kg/day), as well as vitamin D3 (50 IU/day). Histopathological and immunohistochemical analyses were carried out on tissue samples 21 days following exposure to vitamin D3 and TCE. Results: Histopathological examination showed a significant decrease of angiogenesis in the corpus luteum and a substantial degeneration in the ovarian follicles and germinal epithelium in ovarian tissues of TCE group. Also, immunohistochemical examination for MDA immunoreactivity revealed that MDA immunoreactivity was significantly increased in the TCE group compared to the control group. We observed that vitamin D3 administration improved histological parameters, which include follicular degeneration as well as germinal epithelium degeneration, limiting TCE's negative impacts on ovarian tissues. It was also confirmed that MDA immunoreactivity was considerably reduced in ovarian tissues in the TCE + vitamin D3 group. Conclusion: In this study, the significant improvements observed in immunohistochemical and histopathological tissue oxidative stress parameters indicate that treatment with vitamin D3 could be a conservative method to prevent ovarian TCE toxicity.
  1. Bhattacharya P, Keating AF. Impact of environmental exposures on ovarian function and role of xenobiotic metabolism during ovotoxicity. Toxicol Appl Pharmacol. 2012;261(3):227-35. [Crossref]  [PubMed]  [PMC] 
  2. Gilbert KM, Bai S, Barnette D, Blossom SJ. Exposure cessation during adulthood did not prevent immunotoxicity caused by developmental exposure to low-level trichloroethylene in drinking water. Toxicol Sci. 2017;157(2):429-37. [Crossref]  [PubMed]  [PMC] 
  3. Shukla AK, Upadhyay SN, Dubey SK. Current trends in trichloroethylene biodegradation: a review. Crit Rev Biotechnol. 2014;34(2):101-14. [Crossref]  [PubMed] 
  4. Chiu WA, Jinot J, Scott CS, Makris SL, Cooper GS, Dzubow RC, et al. Human health effects of trichloroethylene: key findings and scientific issues. Environ Health Perspect. 2013;121(3):303-11. [Crossref]  [PubMed]  [PMC] 
  5. Lamb JC, Hentz KL. Toxicological review of male reproductive effects and trichloroethylene exposure: assessing the relevance to human male reproductive health. Reprod Toxicol. 2006;22(4):557-63. [Crossref]  [PubMed] 
  6. Stermer AR, Klein D, Wilson SK, Dalaijamts C, Bai CY, Hall SJ, et al. Differential toxicity of water versus gavage exposure to trichloroethylene in rats. Environ Toxicol Pharmacol. 2019;68:1-3. [Crossref]  [PubMed]  [PMC] 
  7. Wu KL, Berger T. Ovarian gene expression is stable after exposure to trichloroethylene. Toxicol Lett. 2008;177(1):59-65. [Crossref]  [PubMed]  [PMC] 
  8. Luo YS, Furuya S, Soldatov VY, Kosyk O, Yoo HS, Fukushima H, et al. Metabolism and toxicity of trichloroethylene and tetrachloroethylene in cytochrome P450 2E1 knockout and humanized transgenic mice. Toxicol Sci. 2018;164(2):489-500. [Crossref]  [PubMed]  [PMC] 
  9. Zhang F, Marty S, Budinsky R, Bartels M, Pottenger LH, Bus J, et al. Analytical methods impact estimates of trichloroethylene's glutathione conjugation and risk assessment. Toxicol Lett. 2018;296:82-94. [Crossref]  [PubMed] 
  10. Bhattacharya P, Keating AF. Ovarian metabolism of xenobiotics. Exp Biol Med (Maywood). 2011;236(7):765-71. [Crossref]  [PubMed]  [PMC] 
  11. Cooper GS, Makris SL, Nietert PJ, Jinot J. Evidence of autoimmune-related effects of trichloroethylene exposure from studies in mice and humans. Environ Health Perspect. 2009;117(5):696-702. [Crossref]  [PubMed]  [PMC] 
  12. Dusso AS, Brown AJ, Slatopolsky E. Vitamin D. Am J Physiol Renal Physiol. 2005;289(1):F8-28. [Crossref]  [PubMed] 
  13. Jones G. Expanding role for vitamin D in chronic kidney disease: importance of blood 25-OH-D levels and extra-renal 1alpha-hydroxylase in the classical and nonclassical actions of 1alpha,25-dihydroxyvitamin D(3). Semin Dial. 2007;20(4):316-24. [Crossref]  [PubMed] 
  14. Hou YF, Gao SH, Wang P, Zhang HM, Liu LZ, Ye MX, et al. 1α,25(OH)₂D₃ suppresses the migration of ovarian cancer SKOV-3 cells through the inhibition of epithelial-mesenchymal transition. Int J Mol Sci. 2016;17(8):1285. [Crossref]  [PubMed]  [PMC] 
  15. Khairy EY, Attia MM. Protective effects of vitamin D on neurophysiologic alterations in brain aging: role of brain-derived neurotrophic factor (BDNF). Nutr Neurosci. 2019;1‐10. [Crossref]  [PubMed] 
  16. Seif AA, Abdelwahed DM. Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats. J Physiol Biochem. 2014;70(3):659-66. [Crossref]  [PubMed] 
  17. Khan S, Priyamvada S, Khan SA, Farooq N, Khan F, Yusufi ANK. Effect of trichloroethylene (TCE) toxicity on the enzymes of carbohydrate metabolism, brush border membrane and oxidative stress in kidney and other rat tissues. Food Chem Toxicol. 2009;47(7):1562-8. [Crossref]  [PubMed] 
  18. Gougeon A. Human ovarian follicular development: from activation of resting follicles to preovulatory maturation. Ann Endocrinol (Paris). 2010;71(3):132-43. [Crossref]  [PubMed] 
  19. Hombach-Klonisch S, Pocar P, Kietz S, Klonisch T. Molecular actions of polyhalogenated arylhydrocarbons (PAHs) in female reproduction. Curr Med Chem. 2005;12(5):599-616. [Crossref]  [PubMed] 
  20. Tsikas D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: analytical and biological challenges. Anal Biochem. 2017;524:13-30. [Crossref]  [PubMed] 
  21. Liang LF, Qi ST, Xian YX, Huang L, Sun XF, Wang WH. Protective effect of antioxidants on the pre-maturation aging of mouse oocytes. Sci Rep. 2017;7(1):1434. [Crossref]  [PubMed]  [PMC] 
  22. Wu KL, Berger T. Trichloroethylene metabolism in the rat ovary reduces oocyte fertilizability. Chem Biol Interact. 2007;170(1):20-30. [Crossref]  [PubMed]  [PMC] 
  23. DuTeaux SB, Berger T, Hess RA, Sartini BL, Miller MG. Male reproductive toxicity of trichloroethylene: sperm protein oxidation and decreased fertilizing ability. Biol Reprod. 2004;70(5):1518-26. [Crossref]  [PubMed] 
  24. Cummings BS, Parker JC, Lash LH. Role of cytochrome P450 and glutathione S-transferase alpha in the metabolism and cytotoxicity of trichloroethylene in rat kidney. Biochem Pharmacol. 2000;59(5):531-43. [Crossref]  [PubMed] 
  25. Lin AMY, Chen KB, Chao PL. Antioxidative effect of vitamin D3 on zinc-induced oxidative stress in CNS. Ann N Y Acad Sci. 2005;1053:319-29. [Crossref]  [PubMed] 
  26. Kwon HJ, Lim JH, Han JT, Lee SB, Yoon WK, Nam KH, et al. The role of vitamin D3 upregulated protein 1 in thioacetamide-induced mouse hepatotoxicity. Toxicol Appl Pharmacol. 2010;248(3):277-84. [Crossref]  [PubMed] 
  27. Sahin HH, Cumbul A, Uslu U, Yilmaz Z, Ercan F, Alican I. The effect of 1,25 dihydroxyvitamin D3 on HCl/Ethanol-induced gastric injury in rats. Tissue Cell. 2018;51:68-76. [Crossref]  [PubMed] 
  28. Tagliaferri S, Porri D, De Giuseppe R, Manuelli M, Alessio F, Cena H. The controversial role of vitamin D as an antioxidant: results from randomised controlled trials. Nutr Res Rev. 2019;32(1):99-105. [Crossref]  [PubMed] 
  29. Heydari M, Ahmadizadeh M, Ahmadi Angali K. Ameliorative effect of vitamin E on trichloroethylene-induced nephrotoxicity in rats. J Nephropathol. 2017;6(3):168-73. [Crossref]  [PubMed]  [PMC] 
  30. Xu H, Tanphaichitr N, Forkert PG, Anupriwan A, Weerachatyanukul W, Vincent R, et al. Exposure to trichloroethylene and its metabolites causes impairment of sperm fertilizing ability in mice. Toxicol Sci. 2004;82(2):590-7. [Crossref]  [PubMed] 
  31. Berger T, Horner CM. In vivo exposure of female rats to toxicants may affect oocyte quality. Reprod Toxicol. 2003;17(3):273-81. [Crossref]  [PubMed] 
  32. Aksak Karamese S, Toktay E, Unal D, Selli J, Karamese M, Malkoc I. The protective effects of beta-carotene against ischemia/reperfusion injury in rat ovarian tissue. Acta Histochem. 2015;117(8):790-7. [Crossref]  [PubMed] 
  33. Ismiyati A, Wiyasa IWA, Hidayati DYN. Protective effect of vitamins C and E on depot-medroxyprogesterone acetate-induced ovarian oxidative stress in vivo. J Toxicol. 2016;2016:3134105. [Crossref]  [PubMed]  [PMC]